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KMID : 1098420060140060371
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Korean Journal of Medicinal Crop Science
2006 Volume.14 No. 6 p.371 ~ p.375
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Effect of Potassium Phosphate on Growth and Ginsenosides Biosynthesis from Ginseng Hairy Root
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In Jun-Gyo
Park Dong-Sik
Lee Bum-Soo
Lee Tae-Hoo
Kim Se-Young
Rho Young-Deok
Cho Dong-Ha
Jin Cheng-Wu
Yang Deok-Chun
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Abstract
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To investigate effects on the growth and ginsenosides accumulation in ginseng hairy root, potassium phosphate was supplemented with 1.25, 2.5, and 5.0 mM concentration in 1/2 MS medium, respectively. Potassium phosphate supplement was increased the biomass and ginsenosides accumulation when it was dose at the concentration of 1.25 mM. And the growth rate of hairy root in the light condition was higher than in dark condition. The highest contents and productivity of ginsenosides were observed at the supplement of 1.25 mM potassium phosphate at the 7th day after the culture onset. Ginseng hairy root cultured in 20 L bioreactor supplemented with 1.25 mM KH_2PO_4 was increased the growth with 1,609 g (F{cdot}W) and ginsenosides content with 11.09 mg than those in control.
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KEYWORD
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Ginsenosides, ginseng, hairy root, potassium phosphate, bioreactor
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